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Hi Guy,

I can agree with Christian re Offloading functionality.

ran the following Display Filter to "ip.addr==10.50.3.53 && ip.addr==10.50.78.91" to display all the SMB traffic. When I enable the Expert Information, we can see a high volume of Duplicate ACK's and Out-of-Order Segments. Not sure if this could also be a possible cause?

From a Win10 perspective, what version are you using (there are various Win10 Builds). When I analysed the SMB2 Response Time, we could see 170x Reads, 46x Create's, 43x Close's, 16x GetInfo to name a few. Also, ensure you have the appropriate SMB features installed (e.g. remove SMB1).

Are you able to perform the same test on another PC to test the extent of the issue.

Hope this info is helpful.