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This is a known issue https://gitlab.com/wireshark/wireshark/-/issues/18553 and only occurs if version 4.0.0 was a clean install and upgraded to 4.0.1 because of the downgrade from Qt6 to Qt5 on Windows.

The workaround is simple: just change(!) the font or font-size in the preferences under Preferences | Appearance | Font and Colors. This will update the preferences file with a font setting (gui.qt.font_name) that is recognized by Qt5.