filtering out protocol, sequence number, and ack using tshark

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Use the "-e" options listed below:

• protocol, -e _ws.col.Protocol
• sequence number, assuming you mean tcp sequence number, -e tcp.seq
• ack, for ack number use -e tcp.ack, for ack flag use -e tcp.flags.ack

In general to find the filter name select the item in the packet details pane and look at the name in parenthesis in the status bar at the bottom. Any column can be used by prefixing the name with "_ws.col.".