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Can't reproduce myself on my Mac, things that come to mind are:

  • Did you have two wireshark windows open and the last one closed did not have the columns in it yet?
  • Did wireshark open with the new profile activated or with the default profile (could e a result too of having multiple wireshark programs running simultaneously and closing them in an unfortunate order).
  • Does wireshark have writing rights to the folder where the profiles are saved?