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[TCP Previous segment not captured ] Wireshark is telling you there is a gap in the TCP sequence numbers.
[ TCP Acked unseen segment ] Wireshark is telling you there is TCP ACK for a sequence number that wasn't in the capture.
The problem is that the sniffer isn't capturing all the packets or there is a split path. The F5 is a low balancer, so a split path is a high probability.
